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Oils and fats testing

Testing for vegetable oils, animal fats, margarines and spreads: fatty acid profile, oxidation indices, contaminants and authenticity.

35 RENAR-accredited testsAnnex no. 1 of 24.04.2026See the table

Oils and fats are products in their own right and key ingredients across the food industry. Their quality depends on the raw material, on refining and on storage conditions, and oxidation starts as soon as they are produced. ICA R&D tests vegetable oils (sunflower, rapeseed, soybean, maize, olive), animal fats (lard, tallow), margarines and spreads, as well as fat extracted from other products.

What we test

Freshness and oxidation are followed through acidity, peroxide value, anisidine value and the Kreis test. The fatty acid profile, obtained by gas chromatography, shows the proportions of saturated, monounsaturated, polyunsaturated and trans fatty acids and the erucic acid content, and helps verify the declared botanical origin of an oil. We complete the characterisation with iodine, saponification and refractive indices, moisture, impurities, cholesterol and tocopherols.

For margarines and spreads we determine total fat, preservatives and sodium chloride. Contaminant testing covers metals, polycyclic aromatic hydrocarbons, dioxins and PCBs, and mycotoxins.

Regulatory framework

  • Regulation (EC) No 178/2002 – general principles of food law
  • Regulation (EU) 2023/915 – maximum levels for contaminants (including erucic acid and PAH)
  • Regulation (EU) 2019/649 – maximum content of trans fat other than trans fat naturally occurring in fat of animal origin
  • Regulation (EC) No 1333/2008 – food additives
  • Regulation (EU) No 1169/2011 – food information to consumers

How we work

Oil samples should be sent in closed containers filled as completely as possible and protected from light and heat, so that oxidation indices do not change in transit. We can recommend the right tests for raw material intake, in-process control or finished product release. Results are issued in a test report; accredited tests are marked as such in the report and are listed in the annex to RENAR accreditation certificate LI 871.

On request, we can give an opinion on whether the fatty acid profile is consistent with the declared origin; the opinion is not covered by the accreditation.

Parameters and determinations

Sensory examination

  • Appearance (external and cross-section) authorised, not accredited
  • Colour authorised, not accredited
  • Texture authorised, not accredited
  • Odour authorised, not accredited
  • Taste authorised, not accredited

Quality and oxidation indices

  • Free fatty acids / acid value authorised, not accredited
  • Peroxide value RENAR accredited
  • Anisidine value subcontracted
  • Iodine value authorised, not accredited
  • Saponification value authorised, not accredited
  • Refractive index authorised, not accredited
  • Kreis test authorised, not accredited
  • Density RENAR accredited
  • Lovibond colour subcontracted

Composition

  • Fatty acid profile (C4–C24), including trans fatty acids RENAR accredited
  • Erucic acid authorised, not accredited
  • Moisture and volatile matter authorised, not accredited
  • Insoluble impurities authorised, not accredited
  • Total fat (margarines, spreads) RENAR accredited
  • Milk fat and non-milk fat subcontracted
  • Cholesterol RENAR accredited
  • Tocopherols authorised, not accredited

Additives

  • Sorbic and benzoic acid RENAR accredited
  • Sodium chloride authorised, not accredited

Contaminants

  • Metals (Pb, Cd, Hg, As, Ni, Cu, Fe and others) authorised, not accredited
  • Polycyclic aromatic hydrocarbons (PAH) subcontracted
  • Dioxins and PCBs subcontracted
  • Mycotoxins authorised, not accredited

Microbiology

Authenticity

  • Fatty acid profile to verify origin subcontracted
  • Species identification (animal fats, DNA) subcontracted

"RENAR accredited" links to the matching row of the accredited-tests table below (RENAR annex no. 1/24.04.2026); "authorised, not accredited" means authorised by ANSVSA (authorisation no. 95/24.09.2025) but outside the accreditation; "subcontracted" means performed by a partner laboratory. An analysis with two labels is accredited only for the matrices and methods in the table. "Not accredited" (non-food products, which need no authorisation) means performed in the ICA laboratory or by a partner laboratory, outside the accreditation.

This list is not exhaustive and not all determinations are accredited. For anything not listed here, please contact our sales team.

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Tell us the product and the tests you need; our sales team will reply with a quote.

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+40 31 410 2020

Samples received after 13:00 are registered and start testing on the next working day.

RENAR-accredited tests

Source: Annex no. 1 to RENAR accreditation certificate LI 871, issued 24/04/2026. Annex (PDF)

RENAR-accredited tests – Oils and fats testing. Source: Annex no. 1 to RENAR accreditation certificate LI 871, issued 24/04/2026.
TestMatrixReference documentAnnex no.
Determination of tin (FAAS) Foodstuffs SR EN 13804:2013; SR EN 13805:2015; SR EN 15764:2010; PS-CH-62 1
Determination of calcium, sodium, potassium and magnesium (FAAS) Foodstuffs SR EN 15505:2008; SR ISO 8070:2008; SR EN ISO 7980:2002; ISO 9964-1:1993; ISO 9964-2:1993; SR EN 13804:2013; SR EN 13805:2015; SR 6182-30:2009; SR 6182-33:1993; SR 6182-32:1993; PS-CH-40 1
Determination of chromium (GFAAS) Foodstuffs SR EN 14083:2003; SR EN 13804:2013; SR EN 13805:2015; PS-CH-62 2
Determination of fatty acid content by gas chromatography Foodstuffs SR EN ISO 12966-2:2017; SR EN ISO 661:2006; SR EN ISO 661:2006/AC:2007; SR EN ISO 12966-4:2015; SR ISO 15885:2008; ISO 14156:2025; PS-CH-30 Ediţia din 10.01.2026 16
Determination of sorbic acid and benzoic acid (HPLC) Margarines SR ISO 22855:2008; SR ISO 9231:2009; Journal of chromatography A, 1073 (2005), 393-397 -Simultaneous determination of preservatives (benzoic acides, sorbic acides, methylparaben and propulbaraben) in foodstuffs using high performance liquid cromatography. (Bahruddin S., Md. Fazlul B., Muhammad I.S., Kamarudzaman A., Mohd. Khairuddin T.) Food Chemistry, 127 (2011) 1294- 1299 - Development of an RP- HPLC method for the simultaneous; determination of benzoic acid, sorbic acid, netamycin and lysizyme in hard and pasta filata cheeses (C. Guarino, F. Fuselli, A. LaMantia, L.Longo); PS-CH-60 Ediţia din 10.01.2022 19
Determination of acesulfame K, aspartame and saccharin by HPLC-DAD Foodstuffs of non-animal origin SR EN 12856:2001; PS-CH-105 Ediția din 10.01.2022 21
Determination of cyclamate by HPLC-DAD Foodstuffs of non-animal origin SR EN 12857:2001; PS-CH-109 Ediția din 10.01.2022 22
Determination of sugars by LC-IR (xylose, fructose, glucose, galactose, sucrose, turanose, maltose, trehalose, lactose, melezitose, maltotriose, raffinose) Foodstuffs and food supplements PS-CH-128, Ediţia din 10.05.2025 23
Determination of acrylamide by LC-MS after acetonitrile extraction/partitioning and dispersive SPE clean-up (modular QuEChERS method) Heat-treated foodstuffs PS-CH-132, Ediţia din 10.02.2023 24
Determination of ochratoxin A by ELISA Agricultural raw materials; foodstuffs derived from them Metoda ELISA – Neogen Veratox 8610; Metoda ELISA RIDASCREEN, cod: R-1312; PS-CH-32 Ediţia din 25.03.2022 26
Determination of protein content by the Dumas method Foodstuffs PS-CH-126, Ediţia din 10.02.2023 33
Determination of peroxide value Animal and vegetable fats and oils SR 13531:2008; PS-CH-100 Ediţia din 10.01.2022 40
Determination of fat content, gravimetric method Butter, edible oil emulsions and spreadable fats SR EN ISO 1736:2009; SR EN ISO 7328:2009; SR EN ISO 2450:2009; SR EN ISO 17189:2005; SR ISO 1444:2008; SR 8613-5:2009; SR 2213-11:2007; SR EN ISO 659:2009; SR 91:2007, cap.14.4; STAS 1227/3-90, cap.12; PS-CH-34 Ediţia din 10.01.2022 47
Determination of lipid content by microwave-assisted extraction Foodstuffs PS-CH-127, Ediţia din 10.02.2023 48
Determination of total, insoluble and soluble dietary fibre Foodstuffs; cereals Metoda AOAC 991.42; Metoda AOAC 985.29; PS-CH-58 52
Mass determinations (net weight, drained weight, mass proportions of components, mass grading) Foodstuffs and feed SR 2213-3:2009; STAS 6516-90; STAS 5951-83; Metoda AOAC 968.30; PS-CH-11 Ediţia din 10.01.2022 53
Determination of cholesterol content Animal fats Metoda spectrofotometrica Enzytec Liquid Cholesterol R-Biopharm (E8320); PS-CH-119 Ediţia din 10.10.2024 60
Determination of volume in the package Liquid foodstuffs PS-CH-10, Ediţia din 10.02.2023 61
Determination of density Foodstuffs PS-CH-07, Ediţia din 10.02.2023 62
Qualitative determination of genetically modified DNA using the SureFood GMO SCREEN 35S/NOS/FMV + IAC kit Foodstuffs Manual kit SureFood GMO SCREEN 35S/NOS/FMV + IAC (R-Biopharm, cod: S2126): Version : December 2016, CONGEN Biotechnologie GmbH, BERLIN, Germany.; PS-MB-117 Ediţia din 20.02.2023 63
Enumeration of microorganisms, colony count at 30 °C Foodstuffs SR EN ISO 4833-1:2014; SR EN ISO 4833-1:2014/A1:2022; SR EN ISO 4833-2:2014; SR EN ISO 4833-2:2014/A1:2022; SR EN ISO 4833-2:2014/AC:2014; PS-MB-01 64
Enumeration of coliforms Foodstuffs SR ISO 4832:2009; PS-MB-02 65
Enumeration of β-glucuronidase-positive Escherichia coli – colony count at 44 °C Foodstuffs SR ISO 16649-2:2007; PS-MB-03 66
Detection of Salmonella Foodstuffs SR EN ISO 6579-1:2017; SR EN ISO 6579-1:2017/A1:2020; PS-MB-04 67
Enumeration of coagulase-positive staphylococci Foodstuffs SR EN ISO 6888-1:2021; SR EN ISO 6888-1:2021/ A1:2023; PS-MB-05 68
Enumeration of presumptive Bacillus cereus – colony count at 30 °C Foodstuffs SR EN ISO 7932:2005; SR EN ISO 7932:2005/ A1:2020; PS-MB-06 69
Enumeration of yeasts and moulds Foodstuffs SR ISO 21527-1:2009; SR ISO 21527-2:2009; PS-MB-07 70
Enumeration of Enterobacteriaceae Foodstuffs PS-MB-21 Ediţia din 10.07.2025 72
Detection of Listeria monocytogenes Foodstuffs SR EN ISO 11290-1:2017; PS-MB-22 73
Enumeration of Listeria monocytogenes Foodstuffs SR EN ISO 11290-2:2017; PS-MB-34 74
Enumeration of mesophilic lactic acid bacteria – colony count at 30 °C Foodstuffs SR ISO 15214:2001; PS-MB-33 75
Enumeration of sulphite-reducing bacteria growing under anaerobic conditions Foodstuffs SR ISO 15213-1:2023; PS-MB-24 76
Detection and enumeration of Campylobacter spp. – colony-count technique Foodstuffs SR EN ISO 10272-2:2017; SR EN ISO 10272-2:2017/A1:2023; PS-MB-32 Ediţia din 10.07.2025 82
Detection of Salmonella spp. by VIDAS UP (SPT) – ELFA Foodstuffs Metoda Biomerieux – VIDAS UP Salmonella (SPT); PS-MB-37 Ediţia din 10.07.2025 83
Calculation of total carbohydrate, available carbohydrate and energy value of food products Foodstuffs Regulamentul (UE) nr. 1169/2011, anexa XIV. FAO Food and Nutrition Paper- Food energy –Methods of analysis and conversion factors; PS-CH-63 Ediţia din 10.01.2024 84

Accreditation covers only the test – matrix – method combinations in the table. The accreditation status of each result is stated on the test report.